Vomiting 5. However, as the microenvironments within the host have variable levels of oxygen, it may be advantageous for bacteria to utilize the nitrite in host tissues. Some of the saprophytic Neisseria spp. The local complications of the infection are salpingitis and pelvic inflammatory disease in the female and epididymitisand urethritis in the male. Immunobiology of gonococcal infection of the human fallopian tube mucosa. It is essential to provide 5-10% CO2. Serogroup C N. meningitidis has been isolated from blood culture of one chimpanzee and from an ocular discharge of a second chimpanzee with purulent conjunctivitis (McClure et al., 1986). For example, mannitol salt agar contains a high concentration of sodium chloride that inhibits the growth of most organisms but permits staphylococci to grow. Escherichia coli. A gram-negative diplococcus grew on 5% sheep blood agar and chocolate agar, had no growth on MacConkey agar, and had no growth on modified Thayer-Martin (MTM) agar. Introduction Thayer-Martin Agar was developed in 1964 for the isolation of N. gonorrhoeae and N. meningitidis. ... Neisseria meningitidis recommended for young adults. N. meningitidis is a fastidious organism, which grows best at 35-37°C with ~5% CO 2 (or in a candle-jar). Bile salts and crystal violet inhibit the growth of Gram positive organisms. Many gonococci carry a plasmid (Pcr) that specifies production of a TEM-1 type of β-lactamase (penicillinase). Meningococcal septicemia, which is characteriz… From: Molecular Medical Microbiology (Second Edition), 2015, Tone Tønjum, Jos van Putten, in Infectious Diseases (Fourth Edition), 2017. Thioglycollate. Neisseria meningitidis, also called N. meningitidis or just meningococcus, is a gram-negative round bacterium that causes meningitis in humans, as well as life-threatening conditions like sepsis and disseminated intravascular coagulation.. Now, N. meningitidis has a thin peptidoglycan layer, so it doesn’t retain the crystal violet dye during Gram staining. Meningococcal infections are a leading cause of morbidity and mortality worldwide, therefore understanding their molecular biology is crucial to develop therapeutics such as vaccines. Colonies remain intact when pushed across the surface of the agar and are unpigmented. The isolate was typed as Y: 14, 19: P1.– and ST3587, and was weakly β-lactamase positive. Neisseria sp. Unlike N. meningitidis and N. gonorrhoeae, the other Neisseria species (except N. lactamica) generally do not grow on selective media such as Thayer–Martin agar. are similar in size, appearance and consistency, except for the saccharolytic Neisseria spp. N. meningitidis (the meningococcus) is a Gram-negative bacterium, and a major causative agent of bacterial meningitis and severe sepsis. PK catalyzes the irreversible conversion of phosphoenolpyruvate to pyruvate with the resulting generation of ATP. "CDC Laboratory Methods for the Diagnosis of Meningitis Caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenza. It is thus possible that intracellular gonococci bind PK to gain an ample source of pyruvate, one of only three carbon sources known to be used by N. gonorrhoeae. The bacteria are transmitted from person-to-person through droplets of respiratory or throat secretions from carriers. They were positive for oxidase and negative for indole production. Nausea 4. Gonococci attach to the microvilli on nonciliated epithelial cells and are internalized by a phagocytic mechanism that results in a membrane-bound vesicle. Figure 27.1. The product of this reaction is then reduced further to nitrous oxide by the membrane‐bound, nitric oxide reductase enzyme, NorB. Experimental meningococcal infection in stump-tail macaques was characterized by a fall in cardiac output, decreased renal blood flow, and increased systemic resistance (Mellins et al., 1972). 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